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Anti-Phospho-p95/NBS1 (S343) antibody [SY0215] LM86075R

WB: 1:500-1:1,000 ICC/IF: 1:50-1:200 IHC-P: 1:50-1:200 IP: Use at an assay dependent concentration.

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Anti-Phospho-p95/NBS1 (S343) antibody [SY0215] 

产品描述

Nijmegen breakage syndrome (NBS) is characterized by extreme radiation sensitivity, chromosomal instability and cancer. These phenotypes are similar to those of ataxia telangiectasia mutated (ATM) disease, where there is a deficiency in a protein kinase that is activated by DNA damage, indicating that the NBS1 (Nibrin) and ATM proteins may participate in common pathways. Nibrin is specifically phosphorylated in response to gamma-radiation, ultraviolet light and exposure to hydroxyurea. The phosphorylation of Nibrin requires catalytically active ATM. ATM physically interacts with and phosphorylates Nibrin on Serine 343 both in vitro and in vivo. Serine 343 is phosphorylated in vitro by ATM and the modification of this residue in vivo is essential for the cellular response to DNA damage. This response includes S-phase checkpoint activation, formation of the NBS1/Mrel1/Rad50 nuclear foci and rescue of hypersensitivity to ionizing radiation.

产品名称Anti-Phospho-p95/NBS1 (S343) antibody [SY0215]

分子量85 kDa

种属反应性Human,Mouse

验证应用WB, ICC/IF, IHC-P, IP

抗体类型重组兔单抗

免疫原Synthetic phospho-peptide corresponding to residues surrounding Ser343 of human p95/NBS1.

偶联Non-conjugated

性能

形态Liquid

浓度1 mg/mL.

存放说明Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.

存储缓冲液1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

亚型IgG

纯化方式Protein A affinity purified.

亚细胞定位Nucleus, PML body, telomere, Chromosome.

数据链接SwissProt: O60934 Human

SwissProt: Q9R207 Mouse

其它名称

AT V1 antibody

AT V2 antibody

ATV antibody

more

应用

  • WB: 1:500-1:1,000
    ICC/IF: 1:50-1:200
    IHC-P: 1:50-1:200
    IP: Use at an assay dependent concentration.

  • Fig1: ICC staining of Phospho-p95/NBS1 (S343) in PC-3M cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibodyfor 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).

    Fig2: Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-Phospho-p95/NBS1 (S343) antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.


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