Anti-TrkB antibody [JE58-22]

产品描述

• Receptor tyrosine kinase involved in the development and the maturation of the central and the peripheral nervous systems through regulation of neuron survival, proliferation, migration, differentiation, and synapse formation and plasticity. Receptor for BDNF/brain-derived neurotrophic factor and NTF4/neurotrophin-4. Alternatively can also bind NTF3/neurotrophin-3 which is less efficient in activating the receptor but regulates neuron survival through NTRK2. Upon ligand-binding, undergoes homodimerization, autophosphorylation and activation. Recruits, phosphorylates and/or activates several downstream effectors including SHC1, FRS2, SH2B1, SH2B2 and PLCG1 that regulate distinct overlapping signaling cascades. Through SHC1, FRS2, SH2B1, SH2B2 activates the GRB2-Ras-MAPK cascade that regulates for instance neuronal differentiation including neurite outgrowth. Through the same effectors controls the Ras-PI3 kinase-AKT1 signaling cascade that mainly regulates growth and survival. Through PLCG1 and the downstream protein kinase C-regulated pathways controls synaptic plasticity. Thereby, plays a role in learning and memory by regulating both short term synaptic function and long-term potentiation. PLCG1 also leads to NF-Kappa-B activation and the transcription of genes involved in cell survival. Hence, it is able to suppress anoikis, the apoptosis resulting from loss of cell-matrix interactions. Isoform GP95-TRKB may also play a role in neutrophin-dependent calcium signaling in glial cells and mediate communication between neurons and glia.

• 产品名称Anti-TrkB antibody [JE58-22]

• 分子量92 kDa

• 种属反应性Mouse,Rat

• 验证应用WB

• 抗体类型重组兔单抗

• 免疫原Recombinant protein within human TrkB aa 100-450.

• 偶联Non-conjugated

性能

• 形态Liquid

• 浓度1 mg/mL.

• 存放说明Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

• 存储缓冲液1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

• 亚型IgG

• 纯化方式Protein A affinity purified.

• 亚细胞定位Cell membrane, Endosome membrane, Early endosome membrane, axon, dendrite, perinuclear region, postsynaptic density.

• 数据链接SwissProt: P15209 Mouse
  SwissProt: Q63604 Rat
  

• 其它名称

  more

• AI848316 antibody

• BDNF tropomyosine receptor kinase B antibody

• BDNF/NT 3 growth factors receptor antibody

应用

• WB: 1:500-1:2,000

• 

  Fig1: Western blot analysis of TrkB on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody ( was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
  Positive control:
  Lane 1: mouse brain tissue lysate
  Lane 2: mouse cerebellum tissue lysate
  Lane 3: rat cerebellum tissue lysate