Anti-SMURF 2 antibody [JU32-34]
产品描述
Smurf1 and Smurf2 (SMAD ubiquitination regulatory factor-1 and 2) are members of the Hect family of proteins, which also includes the ubiquitin (Ub) E3-type ligases Nedd3 and E6-AP. E3 ligases are involved in the enzymatic reactions of the Ub conjugating pathway, which targets proteins for degradation by the 26S proteasome. Within the Ub pathway, the E3 ligases specifically catalyze the transfer of Ub from the Ub-conjugating enzymes to the individual protein substrate. As an E3 ligase, Smurf1 selectively interacts with receptor-regulated SMADs specific to the BMP pathway in order to trigger their ubiquitination and degradation. Smurf2 interacts with receptor-activated Smads (R-Smads), including Smad1, Smad2, and Smad3, but not Smad4. Although Smurf2 localizes to the nucleus, binding to Smad7 induces its export and its recruitment to the activated TGFβ receptor, where it causes degradation of Smad7.
产品名称Anti-SMURF 2 antibody [JU32-34]
分子量86 kDa
种属反应性Human
验证应用WB
抗体类型重组兔单抗
免疫原Recombinant protein
偶联Non-conjugated
性能
形态Liquid
浓度1 mg/mL.
存放说明Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
存储缓冲液1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
亚型IgG
纯化方式Protein A affinity purified.
亚细胞定位Cytoplasm. Membrane. Nucleus.
数据链接Q9HAU4 Human
其它名称
E3 ubiquitin-protein ligase SMURF2 antibody
EC 6.3.2. antibody
hSMURF2 antibody
more
应用
WB: 1:500
Fig1: Western blot analysis of SMURF 2 on A431 cell using anti-SMURF 2 antibody at 1/200 dilution.