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Anti-E2F1 antibody LM8074R

WB: 1:1,000 ICC: 1:100 IP: 1:10 FC: 1:10

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¥ 1500.00 /支
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Anti-E2F1 antibody 

产品描述

The human retinoblastoma gene product appears to play an important role in the negative regulation of cell proliferation. Functional inactivation of Rb can be mediated either through mutation or as a consequence of interaction with DNA tumor virus-encoded proteins. Of all the Rb associations described to date, the identification of a complex between Rb and the transcription factor E2F most directly implicates Rb in regulation of cell proliferation. E2F was originally identified through its role in transcriptional activation of the adenovirus E2 promoter. Sequences homologous to the E2F binding site have been found upstream of a number of genes that encode proteins with putative functions in the G1 and S phases of the cell cycle. E2F-1 is a member of a broader family of transcription regulators including E2F-2, E2F-3, E2F-4, E2F-5, E2F-6 and E2F-7 each of which forms heterodimers with a second protein, DP-1, forming an “active” E2F transcriptional regulatory complex.

产品名称Anti-E2F1 antibody

分子量47kDa

种属反应性Human,Mouse,Rat

验证应用WB,ICC,FC,IP

抗体类型重组兔单抗

免疫原peptide

偶联Non-conjugated

性能

形态Liquid

浓度1 mg/mL.

存放说明Store at -20 ℃. Stable for 12 months from date of receipt

存储缓冲液1*TBS (pH7.4), 0.5%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.

亚型IgG

纯化方式Tissue culture supernatant

亚细胞定位Nucleus

数据链接Q01094

其它名称

DmelCG6376 antibody

Dmel_CG6376 antibody

drosE2F1 antibody

more

应用

  • WB: 1:1,000
    ICC: 1:100
    IP: 1:10
    FC: 1:10

  • Fig1: Anti-E2F1 antibody at 1/1000 dilution Lane 1 : E2F1-DDDDK tag transfected 293T lysate Lane 2 : DDDDK tag-empty plasmid transfected 293T lysate.

    Fig2: Immunofluorescent analysis of HepG2 cells labeling E2F1 with the antibody at 1/100 dilution.


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