Anti-TRP1 antibody [JU36-48] 

产品描述

Tyrosinase (TYR), a type I membrane protein and copper-containing enzyme, is involved in the production of melanin, the primary pigment found in vertebrates. Melanin biogenesis requires the enzymatic activity of TYR, which catalyzes the critical and rate-limiting step of tyrosine hydroxylation in the biosynthesis of melanin. Defects effecting TYR activity result in various forms of albinism. The TYR-related proteins, TRP1 and TRP2, are also specifically expressed in melanocytes, and they likewise contribute to the synthesis of melanin within the melanosomes. The TRPs, including TYR, all share a similar transmembrane region, contain two metal-binding regions and a cysteine-rich epidermal growth factor motif, and are localized in the melanosomal membrane. These proteins, however, have distinct catalytic activity, and they individually contribute to the biosynthesis of melanin biopolymers. The TRPs are believed to exists as a multi-enzyme complex, as these proteins form aggregates together, and the expression of TRP1 also helps stabilize TYR in melanocytes.

产品名称Anti-TRP1 antibody [JU36-48]

分子量72 kDa

种属反应性Human

验证应用WB,ICC,IHC-P,IP

抗体类型重组兔单抗

免疫原Recombinant protein

偶联Non-conjugated

性能

形态Liquid

浓度1 mg/mL.

存放说明Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.

存储缓冲液1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

亚型IgG

纯化方式Protein A affinity purified.

亚细胞定位Membrane.

数据链接P17643 Human

其它名称

5 antibody

5,6 dihydroxyindole 2 carboxylic acid oxidase antibody

6-dihydroxyindole-2-carboxylic acid oxidase antibody

more

应用

• WB: 1:500-1:2,000
  ICC: 1:50-1:100
  IHC-P: 1:50-1:200
  IP: 1:10-1:50

• 

  Fig1: Western blot analysis of TRP1 on melanoma tissue lysate using anti-TRP1 antibody at 1/500 dilution.

  

  Fig2: ICC staining TRP1 in HUVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  

  Fig3: ICC staining TRP1 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  

  Fig4: Immunohistochemical analysis of paraffin-embedded human skin tissue using anti-TRP1 antibody. Counter stained with hematoxylin.