Anti-SV2B antibody [A4C1] 

产品描述

Synaptic vesicle glycoprotein 2B is a protein that in humans is encoded by the SV2B gene.Probably plays a role in the control of regulated secretion in neural and endocrine cells.By similarity (Microbial infection) Receptor for the C.botulinum neurotoxin type A2 (BoNT/A, botA); glycosylation is not essential but enhances the interaction . Probably also serves as a receptor for the closely related C.botulinum neurotoxin type A1.

产品名称Anti-SV2B antibody [A4C1]

分子量Predicted band size: 77 kDa.

种属反应性Human,Rat

验证应用WB,ICC,IHC,FC

抗体类型小鼠单抗

免疫原Recombinant protein within human SV2B aa 400-600.

偶联Non-conjugated

性能

形态Liquid

浓度2 mg/ml.

存放说明Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

存储缓冲液1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

亚型IgG1

纯化方式Protein G affinity purified.

亚细胞定位Cell junction, Cytoplasmic vesicle, Membrane, Synapse.

数据链接SwissProt: Q7L1I2 Human

其它名称

Synaptic vesicle protein 2B antibody

SV2B antibody

应用

• WB: 1:500-1:1,000
  ICC: 1:50-1:100
  IHC: 1:50-1:200
  FC: 1:50-1:100

• 

  Fig1: Western blot analysis of SV2B on rat brain tissue lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (-40, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:5,000 dilution was used for 1 hour at room temperature.

  

  Fig2: ICC staining of SV2B in PANC-1 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (-40, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Mouse IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).

  

  Fig3: ICC staining of SV2B in F9 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (-40, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Mouse IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).

  

  Fig4: Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-SV2B antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (-40, 1/100) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  

  Fig5: Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue using anti-SV2B antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (-40, 1/100) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  

  Fig6: Flow cytometric analysis of SV2B was done on A549 cells. The cells were fixed, permeabilized and stained with the primary antibody (-40, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Mouse IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).