Anti-CD299 antibody [8A1B3] 

产品描述

This gene encodes a transmembrane receptor and is often referred to as L-SIGN because of its expression in the endothelial cells of the lymph nodes and liver. The encoded protein is involved in the innate immune system and recognizes numerous evolutionarily divergent pathogens ranging from parasites to viruses, with a large impact on public health. The protein is organized into three distinct domains: an N-terminal transmembrane domain, a tandem-repeat neck domain and C-type lectin carbohydrate recognition domain. The extracellular region consisting of the C-type lectin and neck domains has a dual function as a pathogen recognition receptor and a cell adhesion receptor by binding carbohydrate ligands on the surface of microbes and endogenous cells. The neck region is important for homo-oligomerization which allows the receptor to bind multivalent ligands with high avidity. Variations in the number of 23 amino acid repeats in the neck domain of this protein are common and have a significant impact on ligand binding ability. This gene is closely related in terms of both sequence and function to a neighboring gene (GeneID 30835; often referred to as DC-SIGN or CD209). DC-SIGN and L-SIGN differ in their ligand-binding properties and distribution. Alternative splicing results in multiple variants.

产品名称Anti-CD299 antibody [8A1B3]

分子量45.4kDa

种属反应性Human

验证应用WB,FC

抗体类型小鼠单抗

免疫原Purified recombinant fragment of human CD299 (AA: extra 237-399) expressed in E. Coli.

偶联Non-conjugated

性能

形态Liquid

浓度1 mg/mL

存放说明Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

存储缓冲液1*PBS with 0.05% sodium azide.

亚型IgG1

纯化方式Protein G affinity purified.

亚细胞定位Secreted and Cell membrane.

数据链接SwissProt: Q9H2X3 Human

其它名称

C type lectin domain family 4, member M antibody

C-type lectin domain family 4 member M antibody

CD209 antigen like antibody

more

应用

• WB: 1:500-1:2,000
  FC: 1:100-1:200

• 

  Fig1: Western blot analysis of CD299 against human CD299 (AA: extra 237-399) recombinant protein. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (-85, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.

  

  Fig2: Western blot analysis of CD299 against HEK293 (1) and CD299 (AA: extra 237-399)-hIgGFc transfected HEK293 (2) cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (-85, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.

  

  Fig3: Western blot analysis of CD299 against L-02 (1), HepG2 (2), BEL-7402 (3), SMMC-7702 (4), and HL-7702 (5) cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (-85, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.

  

  Fig4: Flow cytometric analysis of CD299 was done on MOLT4 cells. The cells were fixed, permeabilized and stained with the primary antibody (-85, 1/100) (green). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated goat anti-Mouse IgG Secondary antibody at 1/500 dilution for 30 minutes. Unlabelled sample was used as a control (cells without incubation with primary antibody; red).