Anti-Alpha-2-macroglobulin antibody [3-G5] 

产品描述

Is able to inhibit all four classes of proteinases by a unique 'trapping' mechanism. This protein has a peptide stretch, called the 'bait region' which contains specific cleavage sites for different proteinases. When a proteinase cleaves the bait region, a conformational change is induced in the protein which traps the proteinase. The entrapped enzyme remains active against low molecular weight substrates (activity against high molecular weight substrates is greatly reduced). Following cleavage in the bait region, a thioester bond is hydrolyzed and mediates the covalent binding of the protein to the proteinase.

产品名称Anti-Alpha-2-macroglobulin antibody [3-G5]

分子量163 kDa

种属反应性Human

验证应用IHC-P,ELISA

抗体类型小鼠单抗

免疫原Recombinant protein within human Alpha-2-macroglobulin aa 950-1200.

偶联Non-conjugated

性能

形态Liquid

浓度2 mg/mL.

存放说明Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.

存储缓冲液1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

亚型IgG2c

纯化方式Protein A affinity purified.

亚细胞定位Secreted.

数据链接P01023

其它名称

A2m antibody

A2MG_HUMAN antibody

Alpha 2 M antibody

more

应用

• IHC-P: 1:50-1:200

• 

  Fig1: Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-Alpha-2-macroglobulin antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (-21, 1/100) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.