Anti-Alpha-2-macroglobulin antibody [8-B10] 

产品描述

Is able to inhibit all four classes of proteinases by a unique 'trapping' mechanism. This protein has a peptide stretch, called the 'bait region' which contains specific cleavage sites for different proteinases. When a proteinase cleaves the bait region, a conformational change is induced in the protein which traps the proteinase. The entrapped enzyme remains active against low molecular weight substrates (activity against high molecular weight substrates is greatly reduced). Following cleavage in the bait region, a thioester bond is hydrolyzed and mediates the covalent binding of the protein to the proteinase.

产品名称Anti-Alpha-2-macroglobulin antibody [8-B10]

分子量163 kDa

种属反应性Human, Mouse, Rat

验证应用ELISA, ICC, IHC-P

抗体类型小鼠单抗

免疫原Recombinant protein within human Alpha-2-macroglobulin aa 950-1200.

偶联Non-conjugated

性能

形态Liquid

浓度2 mg/ml.

存放说明Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

存储缓冲液1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

亚型IgG1

纯化方式Protein A affinity purified.

亚细胞定位Secreted.

数据链接SwissProt: P01023 Human

SwissProt: Q61838 Mouse

SwissProt: P06238 Rat

其它名称

A2m antibody

A2MG_HUMAN antibody

Alpha 2 M antibody

more

应用

• ELISA:1:1,000-1:5,000
  WB:
  IP:
  ICC:1:50
  IF:
  IHC-P:1:50-1:200
  FC:

• 

  Fig1: ICC staining Alpha-2-macroglobulin in H22 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with Alpha-2-macroglobulin monoclonal antibody at a dilution of 1:50 for at least 1 hour at room temperature, washed with PBS. Alexa Fluorc™ 488 Goat anti-Mouse IgG was used as the secondary antibody at 1/100 dilution. The nuclear counter stain is DAPI (blue).

  

  Fig2: Immunohistochemical analysis of paraffin-embedded rat uterus tissue using anti-Alpha-2-macroglobulin antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the antibody (-23) at 1/50 dilution, for 30 minutes at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chrogen. Counter stained with hematoxylin and mounted with DPX.

  

  Fig3: Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-Alpha-2-macroglobulin antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the antibody (-23) at 1/50 dilution, for 30 minutes at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chrogen. Counter stained with hematoxylin and mounted with DPX.

  

  Fig4: Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-Alpha-2-macroglobulin antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the antibody (-23) at 1/50 dilution, for 30 minutes at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chrogen. Counter stained with hematoxylin and mounted with DPX.