Anti-DM1 antibody [A4G3] 

产品描述

Mertansine, also called DM1 (and in some of its forms emtansine), is a thiol-containing maytansinoid that for therapeutic purposes is attached to a monoclonal antibody through reaction of the thiol group with a linker structure to create an antibody-drug conjugate (ADC). Mertansine is a tubulin inhibitor, meaning that it inhibits the assembly of microtubules by binding to tubulin (at the rhizoxin binding site). The monoclonal antibody binds specifically to a structure (usually a protein) occurring in a tumour, thus directing mertansine into this tumour. This concept is called targeted therapy. Trastuzumab emtansine also known as ado-trastuzumab emtansine and sold under the trade name Kadcyla, is an antibody-drug conjugate consisting of the humanized monoclonal antibody trastuzumab (Herceptin) covalently linked to the cytotoxic agent DM1. Trastuzumab alone stops growth of cancer cells by binding to the HER2 receptor, whereas trastuzumab emtansine undergoes receptor-mediated internalization into cells, is catabolized in lysosomes where DM1-containing catabolites are released and subsequently bind tubulin to cause mitotic arrest and cell death. Trastuzumab binding to HER2 prevents homodimerization or heterodimerization (HER2/HER3) of the receptor, ultimately inhibiting the activation of MAPK and PI3K/AKT cellular signalling pathways. Because the monoclonal antibody targets HER2, and HER2 is only over-expressed in cancer cells, the conjugate delivers the cytotoxic agent DM1 specifically to tumor cells.

产品名称Anti-DM1 antibody [A4G3]

验证应用ELISA

抗体类型小鼠单抗

免疫原DM1 coupled with OVA.

偶联Non-conjugated

性能

形态Liquid

浓度2 mg/mL.

存放说明Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

存储缓冲液1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

亚型IgG1

纯化方式Protein G affinity purified.

其它名称

Mertansine antibody

DM1 antibody

Emtansine antibody

应用

• ELISA: 1:5,000-1:20,000

• 

  Fig1: The binding activity of HA600013 with DM1-BSA, MMAF-ADC, DM1-ADC and antibody without conjugaated with DM1. Immobilized protein at 1 μg/ml overnight at 4℃. Then blocked with 1% BSA for 1 hour at 37℃, and incubated with the primary antibody () for 1 hour at 25℃.
  
  Mouse anti-DM1 mAb (HA600014) can bind to DM1 or DM1-ADC but not naked Ab or MMAF-ADC.

  

  Fig2: Down ELISA Binding Assay of anti-DM1 Antibody A4G3 () to ADC-DM1 and DM1-BSA. The mouse mAb works fine with ELISA assay for measuring DM1 derivative ADC.