重组人Ubiquitin-conjugating enzyme E2 T, N-6His, E.coli
英文名 | Recombinant Human Ubiquitin-Conjugating Enzyme E2 T , N-6His, E.coli | ||
相关类别 | 其他重组蛋白 | 储存 | 冷冻(-20℃) |
产品描述
概述
Recombinant Human Ubiquitin-Conjugating Enzyme E2 T is produced by our E.coli expression system and the target gene encoding Met1-Val197 is expressed with a 6His tag at the N-terminus.
使用说明
This material is offered by Sangon Biotech for research, laboratory or further evaluation purposes. NOT FOR HUMAN USE.
技术规格
Tag | N-6His |
种属 | Human |
表达系统 | E.coli |
Accession# | Q9NPD8 |
Source | E.coli |
Formulation_Description | Supplied as a 0.2 μm filtered solution of 50mM HEPES, 150mM NaCl, 2mM DTT, 10% Glycerol, pH 7.5. |
Storage | Store at < -20°C, stable for 6 months after receipt.Please minimize freeze-thaw cycles. |
Purity | Greater than 95% as determined by reducing SDS-PAGE. |
Endotoxin | Less than 0.1 ng/μg (1 EU/μg) as determined by LAL test. |
Background | Ubiquitin-Conjugating Enzyme E2 T (UBE2T) is a ligase that belongs to the Ubiquitin-Conjugating Enzyme family. UBE2T accepts the ATP-dependent ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro, UBE2T is able to catalyze polyubiquitination using all 7 ubiquitin Lys residues, but may prefer 'Lys-11'-, 'Lys-27'-, 'Lys-48'- and 'Lys-63'-linked polyubiquitination. UBE2T is an important factor of the Faconi anemia pathway of DNA damage repair and, upon self-inactivation, may negatively regulate the Faconi pathway. |