Recombinant Tumor Necrosis Factor Receptor Superfamily, Member 14 (TNFRSF14)

肿瘤坏死因子受体超家族成员14(TNFRSF14)重组蛋白

[PROPERTIES]

Source: Prokaryotic expression. Host: E. coli

Residues: Glu45~Glu262

Tags: N-terminal His-Tag

Tissue Specificity: Thymus. Purity: >98%

Traits: Freeze-dried powder

Buffer formulation: 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5%Trehalose and Proclin300. Original Concentration: 200ug/mL

Applications: SDS-PAGE; WB; ELISA; IP; CoIP; Purification; Amine Reactive

Labeling. (May be suitable for use in other assays to be determined by the end user.)

Predicted isoelectric point: 5.5

Predicted Molecular Mass: 25.0kDa

Accurate Molecular Mass: 30kDa as determined by SDS-PAGE reducing conditions. Phenomenon explanation:

The possible reasons that the actual band size differs from the predicted are asfollows:

1. Splice variants: Alternative splicing may create different sized proteins from the same gene.
2. Relative charge: The composition of amino acids may affects the charge of the protein.
 3. Post-translational modification: Phosphorylation, glycosylation, methylation etc.
4. Post-translation cleavage: Many proteins are synthesized as pro-proteins, and then cleaved togive the active form.

5. Polymerization of the target protein: Dimerization, multimerization etc.

[USAGE]

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0mg/mL. Do not vortex.

[STORAGE AND STABILITY]

Storage: Avoid repeated freeze/thaw cycles.

Store at 2-8ºC for one month.

Aliquot and store at -80ºC for 12 months.

Stability Test: The thermal stability is described by the loss rate. The loss ratewas determined by accelerated thermal degradation test, that is, incubate theprotein at 37ºC for 48h, and no obvious degradation and precipitation wereobserved.The loss rate is less than 5% within the expiration date underappropriate storage condition.

[SEQUENCE]

[IDENTIFICATION]

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