Active Interleukin 17C (IL17C) 

白介素17C(IL17C)活性蛋白

[PROPERTIES]

Source: Prokaryotic expression. Host: E. coli

Residues: His19~Val197

Tags: C-terminal His-tag

Purity: >95%

Buffer Formulation: 20mM Tris, 150mM NaCl, pH8.0, containing 0.01% sarcosyl, 5%Trehalose.
Applications: Cell culture; Activity Assays; In vivo assays.
(May be suitable for use in other assays to be determined by the end user.)

Predicted isoelectric point: 8.2

Predicted Molecular Mass: 21.0kDa

Accurate Molecular Mass: 25kDa as determined by SDS-PAGE reducing conditions. Phenomenon explanation:

The possible reasons that the actual band size differs from the predicted are asfollows:

1. Splice variants: Alternative splicing may create different sized proteins from the same gene.
2. Relative charge: The composition of amino acids may affects the charge of the protein.
3. Post-translational modification: Phosphorylation, glycosylation, methylation etc.
4. Post-translation cleavage: Many proteins are synthesized as pro-proteins, and then cleaved togive the active form.

5. Polymerization of the target protein: Dimerization, multimerization etc

[USAGE]

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-0.5mg/mL. Do not vortex

[STORAGE AND STABILITY]

Storage: Avoid repeated freeze/thaw cycles.

Store at 2-8ºC for one month.

Aliquot and store at -80ºC for 12 months.

Stability Test: The thermal stability is described by the loss rate. The loss ratewas determined by accelerated thermal degradation test, that is, incubate theprotein at 37ºC for 48h, and no obvious degradation and precipitation wereobserved. The loss rate is less than 5% within the expiration date underappropriate storage condition

[SEQUENCE]

[ ACTIVITY ]

IL17C (Interleukin-17C) is a T cell-derived cytokine that shares the sequencesimilarity with IL17. IL17C is thought to play a crucial role in innate immunity ofthe epithelium. IL17C binds with affinity to IL17RA. Thus, a binding ELISA assaywas constructed to detect the association of rhIL17C with IL17RA. Briefly, rhIL17C were diluted serially in PBS with 0.1% BSA (pH 7.4). Duplicate samplesof 100uL were then transferred to IL17RA-coated microtiter wells and incubatedfor 2h at 37℃. Wells were washed with PBST and incubated for 1h with anti-IL17pAb, then aspirated and washed 3 times. After incubation with HRP labelledsecondary antibody, wells were aspirated and washed 3 times. With the additionof substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add50µL stop solution to the wells and read at 450nm immediately. The bindingactivity of IL17C with IL17RA was shown in Figure 1 and this effect was in a dosedependent manner.

[IDENTIFICATION]

[ IMPORTANT NOTE ] 

The kit is designed for in vitro and research use only, we will not be responsiblefor any issue if the kit was used in clinical diagnostic or any other procedures.

本产品只适用于科研，不能用于临床诊断。严禁用于临床医疗及其他非科研用途！

以实际收货产品说明书为准，网站说明书仅供参考。