Active Fibronectin Type III Domain Containing Protein 5 (FNDC5)
含Ⅲ型纤连蛋白域蛋白5(FNDC5)活性蛋白
[PROPERTIES]
Source: Prokaryotic expression. Host: E. coli
Residues: Leu2~Ile137
Tags: N-terminal His-tag
Purity: >92%
Endotoxin Level: <1.0EU per 1μg (determined by the LAL method).
Buffer Formulation: 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5% trehalose, and Proclin300.
Applications: Cell culture; Activity Assays; In vivo assays.
(May be suitable for use in other assays to be determined by the end user.)
Predicted isoelectric point: 6.2
Predicted Molecular Mass: 16.6kDa
Accurate Molecular Mass: 18kDa as determined by SDS-PAGE reducing conditions.
[USAGE]
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0mg/mL. Do not vortex
[STORAGE AND STABILITY]
Storage: Avoid repeated freeze/thaw cycles.
Store at 2-8ºC for one month.
Aliquot and store at -80ºC for 12 months.
Stability Test: The thermal stability is described by the loss rate. The loss ratewas determined by accelerated thermal degradation test, that is, incubate theprotein at 37ºC for 48h, and no obvious degradation and precipitation wereobserved. The loss rate is less than 5% within the expiration date underappropriate storage condition
[SEQUENCE]
[ ACTIVITY ]
The protein encoded by FNDC5 can be cleaved into Irisin, which is a myokinelinked to exercise and lean body mass. It was reported that FNDC5 significantlydecreased cell number, migration and viability through apoptosis in malignantMDA-MB-231 cells. Thus MDA-MB-231 cells were seeded overnight at a density of5,000 cells/well, and treated with or without various concentrations of FNDC5 for48h, then MDA-MB-231 cells were observed by inverted microscope and cellviability was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10µL of CCK-8solution was added to each well of the plate, then measure the absorbance at450nm using a microplate reader after incubating the plate for 1-4 hours in at37oC. Cell apoptosis of MDA-MB-231 cells after incubation with FNDC5 for 48h observedby inverted microscope was shown in Figure 1.
[IDENTIFICATION]
本产品只适用于科研,不能用于临床诊断。严禁用于临床医疗及其他非科研用途!
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