Active Interleukin 10 Receptor Beta (IL10Rb)
白介素10受体β(IL10Rb)活性蛋白
[PROPERTIES]
Source: Prokaryotic expression. Host: E. coli
Residues: Leu87~Ala243
Tags: N-terminal His-tag
Purity: >98%
Buffer Formulation: 20mM Tris, 150mM NaCl, pH8.0, containing 0.05% sarcosyl
and 5% trehalose. Applications: Cell culture; Activity Assays; In vivo assays. (May be suitable for use in other assays to be determined by the end user.)
Predicted isoelectric point: 5.0
Predicted Molecular Mass: 19.5kDa
Accurate Molecular Mass: 18kDa as determined by SDS-PAGE reducing conditions.
[USAGE]
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0mg/mL. Do not vortex
[STORAGE AND STABILITY]
Storage: Avoid repeated freeze/thaw cycles.
Store at 2-8ºC for one month.
Aliquot and store at -80ºC for 12 months.
Stability Test: The thermal stability is described by the loss rate. The loss ratewas determined by accelerated thermal degradation test, that is, incubate theprotein at 37ºC for 48h, and no obvious degradation and precipitation wereobserved. The loss rate is less than 5% within the expiration date underappropriate storage condition
[SEQUENCE]
[ ACTIVITY ]
IL10Rb (Interleukin-10 receptor subunit beta) belongs to the cytokine receptorfamily. It is an accessory chain essential for the active interleukin 10 receptorcomplex, which is a cell surface receptor required for the activation of severalcytokines, including IL10. Thus, a binding ELISA assay was conducted to detectthe interaction of IL10Rb and IL10. Briefly, recombinant human IL10RB werediluted serially in PBS, with 0.01%BSA (pH 7.4). Duplicate samples of 100uL werethen transferred to IL10-coated microtiter wells and incubated for 2h at 37°C. Wellswere washed with PBST and incubated for 1h with anti-IL10Rb pAb, thenaspirated and washed 3 times. After incubation with HRP labelled secondaryantibody, wells were aspirated and washed 3 times. With the addition of substratesolution, wells were incubated 15-25 minutes at 37°C. Finally, add 50µL stopsolution to the wells and read at 450nm immediately. The binding activity of IL10Rband IL10 was shown in Figure 1, and this effect was in a dose dependent manner
[IDENTIFICATION]
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