Active Defensin Beta 2 (DEFb2)
防御素β2(DEFb2)活性蛋白
[PROPERTIES]
Source: Prokaryotic expression. Host: E. coli
Residues: Gly24~Pro64
Tags: N-terminal His and GST Tag
Purity: >95%
Endotoxin Level: <1.0EU per 1μg (determined by the LAL method). Buffer Formulation: 20mM Tris, 150mM NaCl, pH8.0, containing 0.01% sarcosyl, 5%Trehalose. Applications: Cell culture; Activity Assays. (May be suitable for use in other assays to be determined by the end user.)
Predicted isoelectric point: 8.0
Predicted Molecular Mass: 34.3kDa
Accurate Molecular Mass: 35kDa as determined by SDS-PAGE reducing conditions
[USAGE]
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0mg/mL. Do not vortex
[STORAGE AND STABILITY]
Storage: Avoid repeated freeze/thaw cycles.
Store at 2-8ºC for one month.
Aliquot and store at -80ºC for 12 months.
Stability Test: The thermal stability is described by the loss rate. The loss ratewas determined by accelerated thermal degradation test, that is, incubate theprotein at 37ºC for 48h, and no obvious degradation and precipitation wereobserved. The loss rate is less than 5% within the expiration date underappropriate storage condition.
[SEQUENCE]
[ ACTIVITY ]
Defensin Beta 2 (DEFb2) known as skin-antimicrobial peptide 1 (SAP1) is acysteine-rich cationic low molecular weight antimicrobial peptide. Defensins form afamily of microbicidal and cytotoxic peptides made by neutrophils. Members of thedefensin family are highly similar in protein sequence. DEFb2 is produced by anumber of epithelial cells and exhibits potent antimicrobial activity againstGram-negative bacteria and Candida, but not Gram-positive S. aureus. Besides, potassium voltage-gated channel, shaker-related subfamily, member 3 (KCNA3)has been identified as an interactor of DEFb2, thus a binding ELISA assay wasconducted to detect the interaction of recombinant human DEFb2 andrecombinant human KCNA3. Briefly, DEFb2 were diluted serially in PBS, with0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred toKCNA3-coated microtiter wells and incubated for 2h at 37℃. Wells were washedwith PBST and incubated for 1h with anti-DEFb2 pAb, then aspirated and washed3 times. After incubation with HRP labelled secondary antibody, wells wereaspirated and washed 3 times. With the addition of substrate solution, wells wereincubated 15-25 minutes at 37℃. Finally, add 50µL stop solution to the wells andread at 450nm immediately. The binding activity of DEFb2 and KCNA3 was shownin Figure 1, and this effect was in a dose dependent manner.
[IDENTIFICATION]
[IMPORTANT NOTE]
The kit is designed for research use only, we will not be responsible for any issue if the kit wasused in clinical diagnostic or any other procedures.
本产品只适用于科研,不能用于临床诊断。严禁用于临床医疗及其他非科研用途!
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