Active Neutrophil Elastase (NE)
中性粒细胞弹性蛋白酶(NE)活性蛋白
[PROPERTIES]
Source: Prokaryotic expression. Host: E. coli
Residues: Ile30~Gln247
Tags: Two N-terminal Tags, His-tag and GST-tag
Purity: >98%
Buffer Formulation: 20mM Tris, 150mM NaCl, pH8.0, containing 0.05% sarcosyl
and 5% trehalose. Applications: Cell culture; Activity Assays. (May be suitable for use in other assays to be determined by the end user.)
Predicted isoelectric point: 8.7
Predicted Molecular Mass: 53.3kDa
Accurate Molecular Mass: 53kDa as determined by SDS-PAGE reducing conditions.
[USAGE]
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0mg/mL. Do not vortex
[STORAGE AND STABILITY]
Storage: Avoid repeated freeze/thaw cycles.
Store at 2-8ºC for one month.
Aliquot and store at -80ºC for 12 months.
Stability Test: The thermal stability is described by the loss rate. The loss ratewas determined by accelerated thermal degradation test, that is, incubate theprotein at 37ºC for 48h, and no obvious degradation and precipitation wereobserved. The loss rate is less than 5% within the expiration date underappropriate storage condition.
[SEQUENCE]
[ ACTIVITY ]
Elastase2 (ELA2), elastase 2 is a serine proteinase in the same family aschymotrypsin and has broad substrate specificity. Secreted by neutrophils andmacrophages during inflammation, it destroys bacteria and host tissue. Theneutrophil form of elastase is 218 amino acids long, with two asparagine-linkedcarbohydrate chains (see glycosylation). Neutrophil elastase may play a role indegenerative and inflammatory diseases by its proteolysis of collagen-IV andelastin of the extracellular matrix. This protein degrades the outer membraneprotein A (OmpA) of E. coli as well as the virulence factors of such bacteria asShigella, Salmonella and Yersinia. Besides, Collagen Type XVII (COL17) has beenidentified as an interactor of ELA2, thus a binding ELISA assay was conducted todetect the interaction of recombinant human COL17 and recombinant humanELA2. Briefly, ELA2 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to COL17-coated microtiterwells and incubated for 2h at 37℃. Wells were washed with PBST and incubatedfor 1h with anti-ELA2 pAb, then aspirated and washed 3 times. After incubationwith HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at37℃. Finally, add 50µL stop solution to the wells and read at 450nm immediately. The binding activity of ELA2 and COL17 was shown in Figure 1, and this effectwas in a dose dependent manner
[IDENTIFICATION]
[IMPORTANT NOTE]
The kit is designed for research use only, we will not be responsible for any issue if the kit wasused in clinical diagnostic or any other procedures.
本产品只适用于科研,不能用于临床诊断。严禁用于临床医疗及其他非科研用途!
以实际收货产品说明书为准,网站说明书仅供参考。