Active Gremlin 1 (GREM1) 

Gremlin 1蛋白(GREM1)活性蛋白

[PROPERTIES]

Source: Prokaryotic expression. Host: E. coli

Residues: Lys26~Asp184

Tags: N-terminal His-tag

Purity: >98%

Endotoxin Level: <1.0EU per 1μg (determined by the LAL method). Buffer Formulation: 20mM Tris, 150mM NaCl, pH8.0, containing 0.05% sarcosyl

and 5% trehalose. Applications: Cell culture; Activity Assays. (May be suitable for use in other assays to be determined by the end user.)

Predicted isoelectric point: 9.0

Predicted Molecular Mass: 24.9kDa

Accurate Molecular Mass: 23kDa as determined by SDS-PAGE reducing conditions

[USAGE]

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0mg/mL. Do not vortex.

[STORAGE AND STABILITY]

Storage: Avoid repeated freeze/thaw cycles.

Store at 2-8ºC for one month.

Aliquot and store at -80ºC for 12 months.

Stability Test: The thermal stability is described by the loss rate. The loss ratewas determined by accelerated thermal degradation test, that is, incubate theprotein at 37ºC for 48h, and no obvious degradation and precipitation wereobserved. The loss rate is less than 5% within the expiration date underappropriate storage condition.

[SEQUENCE]

[ ACTIVITY ] 

Gremlin (GREM1) is an inhibitor in the TGF beta signaling pathway. GREM1 andother BMP antagonists are important in the survival of cancer stroma survival andproliferation in some cancers. The protein expression is found in many cancersand is thought to play important roles in uterine cervix, lung, ovary, kidney, breast, colon, pancreas, and sarcoma carcinomas. To test the effect of GREM1 on inhibitcell proliferation, 3T3 cells were seeded into triplicate wells of 96-well plates at adensity of 5,000 cells/well and allowed to attach, replaced with serum-freeovernight, then the medium was replaced with 2% serum standardDMEM including various concentrations of recombinant human GREM1. Afterincubated for 96h, cells were observed by inverted microscope and cellproliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10µL of CCK-8solution was added to each well of the plate, then the absorbance at 450nm wasmeasured using a microplate reader after incubating the plate for 1-4 hours at37℃. Proliferation of 3T3 cells after incubation with GREM1 for 96h observed byinverted microscope was shown in Figure 1. Cell viability was assessed by CCK-8(Cell Counting Kit-8 ) assay after incubation with recombinant GREM1 for 96h. Theresult was shown in Figure 2. It was obvious that GREM1 significantly increasedcell viability of 3T3 cells.

[IDENTIFICATION]

[IMPORTANT NOTE]

The kit is designed for research use only, we will not be responsible for any issue if the kit wasused in clinical diagnostic or any other procedures.

本产品只适用于科研，不能用于临床诊断。严禁用于临床医疗及其他非科研用途！

以实际收货产品说明书为准，网站说明书仅供参考。