Active Interleukin 20 (IL20)

白介素20(IL20)活性蛋白

[PROPERTIES]

Source: Prokaryotic expression. Host: E. coli

Residues: Leu25~Glu176

Tags: N-terminal His-tag

Purity: >92%

Buffer Formulation: 20mM Tris, 150mM NaCl, pH8.0, containing 0.05% sarcosyl

and 5% trehalose. Applications: Cell culture; Activity Assays; In vivo assays. (May be suitable for use in other assays to be determined by the end user.)

Predicted isoelectric point: 8.9

Predicted Molecular Mass: 21.2kDa

Accurate Molecular Mass: 21kDa as determined by SDS-PAGE reducing conditions.

[USAGE]

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0mg/mL. Do not vortex

[STORAGE AND STABILITY]

Storage: Avoid repeated freeze/thaw cycles.

Store at 2-8ºC for one month.

Aliquot and store at -80ºC for 12 months.

Stability Test: The thermal stability is described by the loss rate. The loss ratewas determined by accelerated thermal degradation test, that is, incubate theprotein at 37ºC for 48h, and no obvious degradation and precipitation wereobserved. The loss rate is less than 5% within the expiration date underappropriate storage condition.

[SEQUENCE]

[ ACTIVITY ]

 IL20 (Interleukin-20) is a cytokine structurally related to interleukin 10, which isproduced by activated keratinocytes and monocytes. It is accepted that IL20regulates proliferation and differentiation of keratinocytes during inflammation, particularly inflammation associated with the skin. Thus, proliferation assay of IL20was conducted using ECV-304 cells. Briefly, ECV-304 cells were seeded intotriplicate wells of 96-well plates at a density of 2,000 cells/well and allowed toattach overnight, then the medium was replaced with serum-free standard1640 prior to the addition of various concentrations of IL20. After incubated for48h, cells were observed by inverted microscope and cell proliferation wasmeasured by Cell Counting Kit-8 (CCK-8). Briefly, 10µL of CCK-8 solution wasadded to each well of the plate, then the absorbance at 450nm was measuredusing a microplate reader after incubating the plate for 1-4 hours at 37°C. Proliferation of ECV-304 cells after incubation with IIL20 for 48h observed byinverted microscope was shown in Figure 1. Cell viability was assessed by CCK-8(Cell Counting Kit-8 ) assay after incubation with human recombinant IL20 for 48h. The result was shown in Figure 2. It was obvious that human IL20 significantlydecreased cell viability of ECV-304 cells.

[IDENTIFICATION]

[IMPORTANT NOTE]

The kit is designed for research use only, we will not be responsible for any issue if the kit wasused in clinical diagnostic or any other procedures.

本产品只适用于科研，不能用于临床诊断。严禁用于临床医疗及其他非科研用途！

以实际收货产品说明书为准，网站说明书仅供参考。