Active S100 Calcium Binding Protein B (S100B) 

S100钙结合蛋白B(S100B)活性蛋白

[PROPERTIES]

Source: Prokaryotic expression. Host: E. coli

Residues: Met1~Glu92

Tags: N-terminal His-tag

Purity: >92%

Endotoxin Level: <1.0EU per 1μg (determined by the LAL method). 

Buffer Formulation: 20mM Tris, 150mM NaCl, pH8.0, containing 0.05% sarcosyl

and 5% trehalose. Applications: Cell culture; Activity Assays. 

(May be suitable for use in other assays to be determined by the end user.)

Predicted isoelectric point: 5.3

Predicted Molecular Mass: 13.5kDa

Accurate Molecular Mass: 14kDa as determined by SDS-PAGE reducing conditions.

[USAGE]

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0mg/mL. Do not vortex

[STORAGE AND STABILITY]

Storage: Avoid repeated freeze/thaw cycles.

Store at 2-8ºC for one month.

Aliquot and store at -80ºC for 12 months.

Stability Test: The thermal stability is described by the loss rate. The loss ratewas determined by accelerated thermal degradation test, that is, incubate theprotein at 37ºC for 48h, and no obvious degradation and precipitation wereobserved.The loss rate is less than 5% within the expiration date underappropriate storage condition

[SEQUENCE]

[ ACTIVITY ] 

Protein S100B is a member of the S100 family. S100 proteins are EF-handcalcium-binding proteins and involved in the regulation of a number of cellularprocesses such as cell cycle progression and differentiation. Experimental resultssuggest that the receptor for advanced glycation end products (RAGE) playsimportant roles in mediating S100 protein-induced cellular signaling. Thus abinding ELISA assay was conducted to detect the interaction of recombinanthuman S100B and recombinant human RAGE. Briefly, S100B were diluted seriallyin PBS, with 0.01%BSA (pH 7.4). Duplicate samples of 100uL were thentransferred to RAGE-coated microtiter wells and incubated for 2h at 37°C. Wellswere washed with PBST and incubated for 1h with anti-S100B mAb, thenaspirated and washed 3 times. After incubation with HRP labelled secondaryantibody, wells were aspirated and washed 3 times. With the addition of substratesolution, wells were incubated 15-25 minutes at 37°C. Finally, add 50µL stopsolution to the wells and read at 450nm immediately. The binding activity of ofS100B and RAGE was shown in Figure 1, and this effect was in a dose dependentmanner.

[IDENTIFICATION]

[IMPORTANT NOTE]

The kit is designed for research use only, we will not be responsible for any issue if the kit wasused in clinical diagnostic or any other procedures.

本产品只适用于科研，不能用于临床诊断。严禁用于临床医疗及其他非科研用途！

以实际收货产品说明书为准，网站说明书仅供参考。