Rutaecarpine 

产品描述

Rutaecarpine is an inhibitor of COX-2 with IC50 of 0.28 μM.

PRODUCT INFORMATION

Chemical Name: Rutaecarpine 

Cat. Number: LM8628MIN

CAS Number: 84-26-4 

Chemical Structure:

体外活性

Rutaecarpine is an indolopyridoquinazolinone alkaloid isolated from Evodia rutaecarpa and related herbs, which has shown a variety of intriguing biological properties such as anti-thrombotic, anticancer, anti-inflammatory and analgesic, anti-obesity and thermoregulatory, vasorelaxing activity, as well as effects on the cardiovascular and endocrine systems. [1]

细胞实验

Rutaecarpine is dissolved in DMSO and diluted with appropriate medium before use. COX-1 and COX-2 cDNA-transfected HEK293 cells are prepared. For measuring inhibitory activity on COX-1 and COX-2 by rutaecarpine, cells in 1 mL of culture medium are seeded into each well of 24-well. After culture for 4 days, the supernatants are removed and 250 mL of fresh medium is added to the cells with or without rutaecarpine. After preincubation for 5 h at 37°C, the cells are further incubated at 37°C for 30 min with 50 mM arachidonic acid. All reactions are stopped by centrifugation at 120 g at 4°C for 5 min. Concentrations of PGE2 in the supernatant are measured[1].

分子式

C18H13N3O

分子量

287.322

别名

Rhetine;Rutacarpine;吴茱萸次碱;Rutecarpine;Rutaecarpine

储存和溶解度

DMSO:23 mg/mL (80.1 mM)

H2O:<1 mg/mL

Ethanol:<1 mg/mL

Powder: -20°C for 3 years

In solvent: -80°C for 2 years

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