Recombinant Beta-1,4-N-Acetyl Galactosaminyl Transferase 2 (b4GALNT2)
β-1,4-N-乙酰半乳糖胺基转移酶2(b4GALNT2)重组蛋白
[ PROPERTIES ]
Residues: Thr324~Ala566
Tags: Two N-terminal Tags, His-tag and T7-tag
Accession: Q8NHY0
Host: E. coli
Subcellular Location: Golgi apparatus, Membrane.
Purity: >95%
Endotoxin Level: <1.0EU per 1μg
(determined by the LAL method).
Formulation: Supplied as lyophilized form in PBS,
pH7.4, containing 5% trehalose, 0.01% sarcosyl.
Predicted isoelectric point: 6.2
Predicted Molecular Mass: 31.3kDa
Applications: SDS-PAGE; WB; ELISA; IP.
(May be suitable for use in other assays to be determined by the end user.)
[ USAGE ]
Reconstitute in sterile PBS, pH7.2-pH7.4
[ STORAGE AND STABILITY ]
Storage: Avoid repeated freeze/thaw cycles.
Store at 2-8ºC for one month.
Aliquot and store at -80ºC for 12 months.
Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37ºC for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.
[ SEQUENCES ]
The sequence of the target protein is listed below.
TIATKTF LRPHKLMIML RSIREYYPDL TVIVADDSQK PLEIKDNHVE YYTMPFGKGWFAGRNLAISQ VTTKYVLWVD DDFLFNEETK IEVLVDVLEK TELDVVGGSV LGNVFQFKLLLEQSENGACL HKRMGFFQPL DGFPSCVVTS GVVNFFLAHT ERLQRVGFDP RLQRVAHSEFFIDGLGTLLV GSCPEVIIGH QSRSPVVDSE LAALEKTYNT YRSNTLTRVQ FKLALHYFKNHLQCAA
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