Recombinant Brain Derived Neurotrophic Factor (BDNF)
Source: Prokaryotic expression
Tags: N-terminal His Tag
Subcellular Location: Secreted
Purity: > 95%
Traits: Freeze-dried powder
Buffer formulation: 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT,
0.01% SKL, 5% Trehalose.
Original Concentration: 200µg/mL
Applications: Positive Control; Immunogen; SDS-PAGE; WB.
(May be suitable for use in other assays to be determined by the end user.)
Predicted isoelectric point: 8.8
Predicted Molecular Mass: 27.4kDa
Accurate Molecular Mass: 32kDa as determined by SDS-PAGE reducing conditions.
The possible reasons that the actual band size differs from the predicted are as follows:
1.Splice variants: Alternative splicing may create different sized proteins from the same gene.
2. Relative charge: The composition of amino acids may affects the charge of the protein.
3. Post-translational modification: Phosphorylation, glycosylation, methylation etc.
4. Post-translation cleavage: Many proteins are synthesized as pro-proteins, and then cleavedto give the active form.
5. Polymerization of the target protein: Dimerization, multimerization etc
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do notvortex.
[STORAGE AND STABILITY]
The kit is designed for research use only, we will not be responsible for any issue if the kit wasused in clinical diagnostic or any other procedures.