Recombinant Flavin Containing Monooxygenase 2, Non Functional (FMO2) 

含黄素单加氧酶2(FMO2)重组蛋白

[ PROPERTIES ]

Residues: Arg116~Val324

Tags: Two N-terminal Tags, His-tag and T7-tag

Accession: Q6IRI9

Host: E. coli

Subcellular Location: Microsome Membrane.

Endoplasmic Reticulum Membrane.

Purity: >95%

Endotoxin Level: <1.0EU per 1μg

(determined by the LAL method).

Formulation: Supplied as lyophilized form in 20mM Tris,

150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT,

0.01% sarcosyl, 5% trehalose, and preservative.

Predicted isoelectric point: 8.6

Predicted Molecular Mass: 27.8kDa

Applications: SDS-PAGE; WB; ELISA; IP.

(May be suitable for use in other assays to be determined by the end user.)

[ USAGE ]

Reconstitute in sterile ddH₂O

[ STORAGE AND STABILITY ]

Storage: Avoid repeated freeze/thaw cycles.

Store at 2-8ºC for one month.

Aliquot and store at -80ºC for 12 months.

Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37ºC for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

[ SEQUENCES ]

The sequence of the target protein is listed below.

RPDFA SSGQWDVYVQ SNGKEQRAVF DAVMVCSGHH IQPHLPLKSF PGIERFQGQYFHSRQYKHPV GYEGKRILVV GIGNSAADIA SELSKRAAQV FVSTRHGSWV LSRISEDGYPWDMVFHTRFS SM LRNVLPRT VVKWMMERQM NRWFNHENYG LVPQNKYLMKEPVLNDDLPS RLLYGAIKVK TRVKELTETA VVFEDGTVEE DVDV

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