Active Fatty Acid Binding Protein 4 (FABP4)

脂肪酸结合蛋白 4(FABP4)活性蛋白

[PROPERTIES]

Source: Eukaryotic expression. Host: 293F cell

Residues: Cys2~Ala132

Tags: N-terminal His-tag

Purity: >95%

Endotoxin Level: <1.0EU per 1μg (determined by the LAL method). Buffer Formulation: 10mM PBS, pH7.6, containing 5% trehalose. Original Concentration: 25ug/mL

Applications: Cell culture; Activity Assays. (May be suitable for use in other assays to be determined by the end user.)

Predicted isoelectric point: 6.8

Predicted Molecular Mass: 16.2kDa

Accurate Molecular Mass: 16kDa as determined by SDS-PAGE reducing conditions

[USAGE]

Reconstitute in ddH₂O to a concentration of 0.1-0.5 mg/mL. Do not vortex.

[STORAGE AND STABILITY]

Storage: Avoid repeated freeze/thaw cycles.

Store at 2-8ºC for one month.

Aliquot and store at -80ºC for 12 months.

Stability Test: The thermal stability is described by the loss rate. The loss ratewas determined by accelerated thermal degradation test, that is, incubate theprotein at 37ºC for 48h, and no obvious degradation and precipitation wereobserved. The loss rate is less than 5% within the expiration date underappropriate storage condition.

[SEQUENCE]

[ ACTIVITY ] 

Fatty Acid Binding Protein 4, Adipocyte (FABP4) encodes the fatty acid bindingprotein found in adipocytes. Fatty acid binding proteins are a family of small, highlyconserved, cytoplasmic proteins that bind long-chain fatty acids and otherhydrophobic ligands. It is thought that FABPs roles include fatty acid uptake,transport, and metabolism. Besides, Actin Beta (ACTb) has been identified as aninteractor of FABP4, thus a binding ELISA assay was conducted to detect theinteraction of recombinant human FABP4 and recombinant human ACTb. Briefly, FABP4 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samplesof 100μL were then transferred to ACTb-coated microtiter wells and incubated for2h at 37℃. Wells were washed with PBST and incubated for 1h with anti-FABP4pAb, then aspirated and washed 3 times. After incubation with HRP labelledsecondary antibody, wells were aspirated and washed 3 times. With the addition ofsubstrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50µLstop solution to the wells and read at 450nm immediately. The binding activity ofFABP4 and ACTb was shown in Figure 1, and this effect was in a dose dependentmanner

[IDENTIFICATION]

[IMPORTANT NOTE]

The kit is designed for research use only, we will not be responsible for any issue if the kit wasused in clinical diagnostic or any other procedures.

本产品只适用于科研，不能用于临床诊断。严禁用于临床医疗及其他非科研用途！

以实际收货产品说明书为准，网站说明书仅供参考。