Active Interleukin 25 (IL25)
白介素25(IL25)活性蛋白
[PROPERTIES]
Source: Prokaryotic expression. Host: E. coli
Residues: Met28~Gly177
Tags: N-terminal His-tag
Purity: >98%
Buffer Formulation: 20mM Tris, 150mM NaCl, pH8.0, containing 0.05% sarcosyl
and 5% trehalose. Applications: Cell culture; Activity Assays; In vivo assays. (May be suitable for use in other assays to be determined by the end user.)
Predicted isoelectric point: 8.6
Predicted Molecular Mass: 18.5kDa
Accurate Molecular Mass: 21kDa as determined by SDS-PAGE reducing conditions.
[USAGE]
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0mg/mL. Do not vortex
[STORAGE AND STABILITY]
Storage: Avoid repeated freeze/thaw cycles.
Store at 2-8ºC for one month.
Aliquot and store at -80ºC for 12 months.
Stability Test: The thermal stability is described by the loss rate. The loss ratewas determined by accelerated thermal degradation test, that is, incubate theprotein at 37ºC for 48h, and no obvious degradation and precipitation wereobserved.The loss rate is less than 5% within the expiration date underappropriate storage condition
[SEQUENCE]
[ ACTIVITY ]
IL25 (Interleukin-25), also known as IL17E, is reported to induce NF-κB activation, and stimulate the production of IL-8. Besides, this cytokine shares the sequencesimilarity with IL17, which is considered as a ligand for IL17RA. Thus, a bindingELISA assay was conducted to detect the association of recombinant human IL25with IL17RA. Briefly, IL25 were diluted serially in PBS with 0.1%BSA (pH 7.4). Duplicate samples of 100uL were then transferred to IL17RA-coated microtiterwells and incubated for 2h at 37°C. Wells were washed with PBST and incubatedfor 1h with anti-IL25 pAb, then aspirated and washed 3 times. After incubation withHRP labelled secondary antibody, wells were aspirated and washed 3 times. Withthe addition of substrate solution, wells were incubated 15-25 minutes at 37°C. Finally, add 50µL stop solution to the wells and read at 450nm immediately. Thebinding activity of IL25 with IL17RA was shown in Figure 1 and this effect was in adose dependent manner.
[IDENTIFICATION]
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