Active C Reactive Protein (CRP)  

C反应蛋白(CRP)活性蛋白

[PROPERTIES]

Source: Natural Extract

Host: Human (Plasma)

Purity: >98% as determined by SDS-PAGE. Endotoxin Level: <1.0EU per 1μg (determined by the LAL method). Purification Methods: Salt co-precipitation and ionic-Exchange chromatography. Traits: Freeze-dried powder

Buffer Formulation: 10mM PBS, pH7.4, containing 5% trehalose. Original Concentration: 200µg/mL

Applications: Positive Control; Immunogen; SDS-PAGE; WB. 

(May be suitable for use in other assays to be determined by the end user.)

Predicted isoelectric point: 5.5

Accurate Molecular Mass: 25.0kDa

Observe Molecular Mass: 25kDa

[USAGE]

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0mg/mL. Do not vortex

[STORAGE AND STABILITY]

Storage: Avoid repeated freeze/thaw cycles.

Store at 2-8ºC for one month.

Aliquot and store at -80ºC for 12 months.

Stability Test: The thermal stability is described by the loss rate. The loss ratewas determined by accelerated thermal degradation test, that is, incubate theprotein at 37ºC for 48h, and no obvious degradation and precipitation wereobserved.The loss rate is less than 5% within the expiration date underappropriate storage condition

[SEQUENCE]

[ ACTIVITY ] 

C Reactive Protein (CRP) is an annular (ring-shaped), pentameric protein, amember of the pentraxin family of proteins. It is an acute-phase protein of hepaticorigin that increases following interleukin-6 secretion by macrophages and T cells.Its physiological role is to bind to lysophosphatidylcholine expressed on thesurface of dead or dying cells (and some types of bacteria) in order to activate thecomplement system via C1q. CRP is synthesized by the liver in response tofactors released by macrophages and fat cells (adipocytes). Besides, RibosomalProtein L23A (RPL23A) has been identified as an interactor of CRP, thus a bindingELISA assay was conducted to detect the interaction of recombinant human CRPand recombinant human RPL23A. Briefly, CRP were diluted serially in PBS, with0.01% BSA (pH 7.4). Duplicate samples of 100μL were then transferred toRPL23A-coated microtiter wells and incubated for 2h at 37℃. Wells were washedwith PBST and incubated for 1h with anti-CRP pAb, then aspirated and washed 3times. After incubation with HRP labelled secondary antibody, wells were aspiratedand washed 3 times. With the addition of substrate solution, wells were incubated15-25 minutes at 37℃. Finally, add 50µL stop solution to the wells and read at450nm immediately. The binding activity of CRP and RPL23A was shown in Figure1, and this effect was in a dose dependent manner

[IDENTIFICATION]

[IMPORTANT NOTE]

The kit is designed for research use only, we will not be responsible for any issue if the kit wasused in clinical diagnostic or any other procedures.

本产品只适用于科研，不能用于临床诊断。严禁用于临床医疗及其他非科研用途！

以实际收货产品说明书为准，网站说明书仅供参考。