Active Fibroblast Growth Factor 3 (FGF3)

成纤维细胞生长因子3(FGF3)活性蛋白

[PROPERTIES]

Source: Prokaryotic expression. Host: E. coli

Residues: Ala18~His239

Tags: N-terminal His-tag

Purity: >95%

Endotoxin Level: <1.0EU per 1μg (determined by the LAL method). 

Buffer Formulation:100mMNaHCO₃, 500mM NaCl, pH8.3, containing 0.01%sarcosyl, 5%Trehalose. 

Applications: Cell culture; Activity Assays. 

(May be suitable for use in other assays to be determined by the end user.)

Predicted isoelectric point: 11.0

Predicted Molecular Mass: 28.7kDa

Accurate Molecular Mass: 33kDa as determined by SDS-PAGE reducing conditions.

[USAGE]

Reconstitute in ddH₂O o a concentration of 0.1-1.0 mg/mL. Do not vortex.

[STORAGE AND STABILITY]

Storage: Avoid repeated freeze/thaw cycles.

Store at 2-8ºC for one month.

Aliquot and store at -80ºC for 12 months.

Stability Test: The thermal stability is described by the loss rate. The loss ratewas determined by accelerated thermal degradation test, that is, incubate theprotein at 37ºC for 48h, and no obvious degradation and precipitation wereobserved. The loss rate is less than 5% within the expiration date underappropriate storage condition

[SEQUENCE]

[ ACTIVITY ]

Fibroblast Growth Factor 3 (FGF3) also known as INT-2 proto-oncogene protein isa member of the fibroblast growth factor family. FGF family members possessbroad mitogenic and cell survival activities and are involved in a variety ofbiological processes including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. Besides, FibroblastGrowth Factor Receptor 2 (FGFR2) has been identified as an interactor of FGF3,thus a binding ELISA assay was conducted to detect the interaction ofrecombinant human FGF3 and recombinant human FGFR2. Briefly, FGF3 werediluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100μL werethen transferred to FGFR2-coated microtiter wells and incubated for 2h at 37℃. Wells were washed with PBST and incubated for 1h with anti-Fibroblast GrowthFactor Receptor 2 (FGFR2) pAb, then aspirated and washed 3 times. Afterincubation with HRP labelled secondary antibody, wells were aspirated andwashed 3 times. With the addition of substrate solution, wells were incubated15-25 minutes at 37℃. Finally, add 50µL stop solution to the wells and read at450nm immediately. The binding activity of FGF3 and FGFR2 was shown in Figure1, and this effect was in a dose dependent manner.

[IDENTIFICATION]

[ IMPORTANT NOTE ] 

The kit is designed for in vitro and research use only, we will not be responsiblefor any issue if the kit was used in clinical diagnostic or any other procedures.

本产品只适用于科研，不能用于临床诊断。严禁用于临床医疗及其他非科研用途！

以实际收货产品说明书为准，网站说明书仅供参考。