Active Interleukin 1 Receptor Antagonist (IL1RA) 

白介素1受体拮抗剂(IL1RA)活性蛋白

[PROPERTIES]

Source: Eukaryotic expression. Host: 293F cell

Residues: Arg26~Glu177

Tags: N-terminal His-tag

Purity: >95%

Endotoxin Level: <1.0EU per 1μg (determined by the LAL method).
Buffer Formulation: 10mM PBS, pH7.6, containing 5% trehalose.
Applications: Cell culture; Activity Assays.
 (May be suitable for use in other assays to be determined by the end user.)

Predicted isoelectric point: 5.4

Predicted Molecular Mass: 18.7kDa

Accurate Molecular Mass: 22/25/27kDa as determined by SDS-PAGE reducing

conditions. Phenomenon explanation:

The possible reasons that the actual band size differs from the predicted are asfollows:

1. Splice variants: Alternative splicing may create different sized proteins from the same gene.
2. Relative charge: The composition of amino acids may affects the charge of the protein.
3. Post-translational modification: Phosphorylation, glycosylation, methylation etc.
4. Post-translation cleavage: Many proteins are synthesized as pro-proteins, and then cleaved togive the active form.

5. Polymerization of the target protein: Dimerization, multimerization etc

[USAGE]

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0mg/mL. Do not vortex

[STORAGE AND STABILITY]

Storage: Avoid repeated freeze/thaw cycles.

Store at 2-8ºC for one month.

Aliquot and store at -80ºC for 12 months.

Stability Test: The thermal stability is described by the loss rate. The loss ratewas determined by accelerated thermal degradation test, that is, incubate theprotein at 37ºC for 48h, and no obvious degradation and precipitation wereobserved. The loss rate is less than 5% within the expiration date underappropriate storage condition.

[SEQUENCE]

[ ACTIVITY ] 

IL-1RA is an agent that binds non-productively to the cell surface interleukin-1receptor (IL-1R), the same receptor that binds interleukin 1 (IL-1), preventing IL-1from sending a signal to that cell. Besides, Interleukin 1 Alpha (IL-1a) has beenidentified as an interactor of IL-1RA, thus a binding ELISA assay was conducted todetect the interaction of recombinant human IL-1RA and recombinant human IL-1a. Briefly, IL1RA were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicatesamples of 100μL were then transferred to IL-1a-coated microtiter wells andincubated for 2 h at 37℃. Wells were washed with PBST and incubated for 1 hwith anti-IL-1RA pAb, then aspirated and washed 3 times. After incubation withHRP labelled secondary antibody, wells were aspirated and washed 3 times. Withthe addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50µL stop solution to the wells and read at 450nm immediately. Thebinding activity of IL-1RA and IL-1a was shown in Figure 1, and this effect was in adose dependent manner.

[IDENTIFICATION]

[IMPORTANT NOTE]

The kit is designed for research use only, we will not be responsible for any issue if the kit wasused in clinical diagnostic or any other procedures.

本产品只适用于科研，不能用于临床诊断。严禁用于临床医疗及其他非科研用途！

以实际收货产品说明书为准，网站说明书仅供参考。