Active Apolipoprotein A1 (APOA1)
载脂蛋白A1(APOA1)活性蛋白
[PROPERTIES]
Source: Prokaryotic expression. Host: E. coli
Residues: Gln122~Gln267
Tags: N-terminal His-tag
Purity: >95%
Endotoxin Level: <1.0EU per 1μg (determined by the LAL method).
Buffer Formulation: 20mM Tris, 150mM NaCl, pH8.0, containing 0.05% sarcosyl
and 5% trehalose. Applications: Cell culture; Activity Assays.
(May be suitable for use in other assays to be determined by the end user.)
Predicted isoelectric point: 6.4
Predicted Molecular Mass: 18.5kDa
Accurate Molecular Mass: 17kDa as determined by SDS-PAGE reducing conditions.
[USAGE]
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0mg/mL. Do not vortex
[STORAGE AND STABILITY]
Storage: Avoid repeated freeze/thaw cycles.
Store at 2-8ºC for one month.
Aliquot and store at -80ºC for 12 months.
Stability Test: The thermal stability is described by the loss rate. The loss ratewas determined by accelerated thermal degradation test, that is, incubate theprotein at 37ºC for 48h, and no obvious degradation and precipitation wereobserved.The loss rate is less than 5% within the expiration date underappropriate storage condition
[SEQUENCE]
[ ACTIVITY ]
Apolipoprotein A1 (APOA1) is the major protein component of HDL particles inplasma. It is a cofactor for lecithin cholesterolacyltransferase (LCAT) which isresponsible for the formation of most plasma cholesteryl esters. ApoA1 was alsoisolated as a prostacyclin (PGI2) stabilizing factor, and thus may have ananticlotting effect. ApoA1 is often used as a biomarker for prediction ofcardiovascular diseases. Besides, Haptoglobin (Hpt) has been identified as aninteractor of APOA1, thus a binding ELISA assay was conducted to detect theinteraction of recombinant human APOA1 and recombinant human Hpt. Briefly, APOA1 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samplesof 100uL were then transferred to Hpt-coated microtiter wells and incubated for 2hat 37℃. Wells were washed with PBST and incubated for 1h with anti-APOA1 pAb,then aspirated and washed 3 times. After incubation with HRP labelled secondaryantibody, wells were aspirated and washed 3 times. With the addition of substratesolution, wells were incubated 15-25 minutes at 37 ℃ . Finally, add 50µL stopsolution to the wells and read at 450nm immediately. The binding activity ofAPOA1 and Hpt was shown in Figure 1, and this effect was in a dose dependentmanner.
[IDENTIFICATION]
[IMPORTANT NOTE]
The kit is designed for research use only, we will not be responsible for any issue if the kit wasused in clinical diagnostic or any other procedures.
本产品只适用于科研,不能用于临床诊断。严禁用于临床医疗及其他非科研用途!
以实际收货产品说明书为准,网站说明书仅供参考。